LRA media is intended to be used as a lipid sorbent in the
production of biopharmaceuticals. LRA media is also used
in sample preparation and solid phase extraction. LRA media
provides outstanding performance in terms of lipid capacity,
filterability, product recovery, and scalability.
LRA media is a synthetic calcium silicate hydrate. It is
composed of 32% calcium oxide, 48% silicon dioxide, and
residual levels of sodium, magnesium, and iron.
The specificity of LRA media for lipids and lipoproteins
is high, allowing for removal of contaminating lipids from
biological streams without an adverse effect on non-lipids.
To demonstrate this, we measured the potential for adsorption
of various plasma proteins to LRA media. Following the incubation
with cryo-reduced source plasma (samples were mixed for
three hours at room temperature) soluble protein was measured
by nephelometry. The results show that LRA media is specific
for lipids and lipid associated proteins within the normal
range of additions. Very little non-specific binding of
non-lipid associated proteins was observed (Table
Pressure Flow Curves
LRA media generates acceptable differential pressures at
moderately high flux rates (Figure
3). A bed height of 1cm generates differential pressures
of 25 psi at a flux rate of 2400 LMH (240 cm/hr linear velocity).
When the bed height is increased to 4 cm, the maximum flux
rate is 600LMH (60 cm/hr linear velocity) for the same differential
pressure. This linear relationship between pressure and
bed height must be considered when designing a process using
Purity Filtration, Sorbents,
Gas-Liquid Chromatography, Sample
Notes, SDS - NAFTA,
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